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Hyphenated Techniques

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Natural Products Isolation

Part of the book series: Methods in Biotechnology ((MIBT,volume 20))

Summary

The technique developed from the coupling of a separation technique and an on-line spectroscopic detection technology is known as hyphenated technique. The remarkable improvements in hyphenated analytical methods over the last two decades have significantly broadened their applications in the analysis of biomaterials, especially natural products. In this chapter, recent advances in the applications of various hyphenated techniques, e.g., GC-MS, LC-PDA, LC-MS, LC-FTIR, LC-NMR, LC-NMR-MS, and CE-MS, in the context of preisolation analyses of crude extracts or fraction from various natural sources, isolation and on-line detection of natural products, chemotaxonomic studies, chemical fingerprinting, quality control of herbal products, dereplication of natural products, and metabolomic studies are discussed with appropriate examples. Particular emphasis is given on the hyphenated techniques that involve liquid chromatography, as the separation tool.

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Author information

Authors and Affiliations

  1. Pharmaceutical Biotechnology Research Group, School of Biomedical Sciences, University of Ulster at Coleraine, Coleraine, Northern Ireland, UK

    Satyajit D. Sarker

  2. School of Life Sciences, The Robert Gordon University, Aberdeen, Scotland, UK

    Lutfun Nahar

Authors
  1. Satyajit D. Sarker
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  2. Lutfun Nahar
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Editor information

Editors and Affiliations

  1. Pharmaceutical Biotechnology Research Group school of Biomedical Sciences, University of Ulster at Coleraine, Coleraine, Northern Ireland, UK

    Satyajit D. Sarker

  2. Molecular Nature Limited Plas Gogerddan, Aberystwyth, Wales, UK

    Zahid Latif

  3. Phytochemistry Research Lab Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, Scotland, UK

    Alexander I. Gray

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© 2006 Humana Press Inc., Totowa,NJ

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Sarker, S.D., Nahar, L. (2006). Hyphenated Techniques. In: Sarker, S.D., Latif, Z., Gray, A.I. (eds) Natural Products Isolation. Methods in Biotechnology, vol 20. Humana Press. https://doi.org/10.1385/1-59259-955-9:233

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  • DOI: https://doi.org/10.1385/1-59259-955-9:233

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-447-0

  • Online ISBN: 978-1-59259-955-4

  • eBook Packages: Springer Protocols

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