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Natural Products Isolation pp 185–211Cite as

Separation by High-Speed Countercurrent Chromatography

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Part of the book series: Methods in Biotechnology ((MIBT,volume 20))

Summary

High-speed countercurrent chromatography provides the natural product chemist with a high-resolution, separatory method that is uniquely applicable to sensitive (unstable) compounds and virtually allows quantitative recovery of the load sample. Different instruments use several means of retaining a stationary liquid phase. The solvent system can be chosen to optimize the separatory power and the number of systems available is limitless. Several examples are provided to illustrate the power of the method and to guide the chemist in the choice of an appropriate system.

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Notes

  1. 1.

    *

    In an aberration, the US Department of Patents and Trademarks allowed Bristol Myers Squibb to trademark the name taxol despite the fact that this name had been given to the natural product many years earlier by its discoverer and had been used in the literature consistently. The current approved generic name for the compound is paclitaxel.

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Author information

Authors and Affiliations

  1. Ecopia BioSciences Inc., Frederick Banting, Saint Laurent, Quebec, Canada

    James B. McAlpine & Patrick Morris

Authors
  1. James B. McAlpine
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  2. Patrick Morris
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Editor information

Editors and Affiliations

  1. Pharmaceutical Biotechnology Research Group school of Biomedical Sciences, University of Ulster at Coleraine, Coleraine, Northern Ireland, UK

    Satyajit D. Sarker

  2. Molecular Nature Limited Plas Gogerddan, Aberystwyth, Wales, UK

    Zahid Latif

  3. Phytochemistry Research Lab Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, Scotland, UK

    Alexander I. Gray

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© 2006 Humana Press Inc., Totowa,NJ

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McAlpine, J.B., Morris, P. (2006). Separation by High-Speed Countercurrent Chromatography. In: Sarker, S.D., Latif, Z., Gray, A.I. (eds) Natural Products Isolation. Methods in Biotechnology, vol 20. Humana Press. https://doi.org/10.1385/1-59259-955-9:185

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  • DOI: https://doi.org/10.1385/1-59259-955-9:185

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-447-0

  • Online ISBN: 978-1-59259-955-4

  • eBook Packages: Springer Protocols

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