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Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2

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Calcium Signaling Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 312))

Abstract

An elevation in intracellular calcium concentration ([Ca2+]i) acts to trigger a range of cellular events including neurotransmitter release, muscle contraction, and oocyte fertilization (1,2). The pattern of elevation in [Ca2+]i and response to that elevation is dependent on the agonist and the cell type.

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Author information

Authors and Affiliations

  1. Department of Child Health, Division of Anaestheisa, Critical Care, and Pain Management, Leicester Royal Infirmary, University of Leicester, Leicester, UK

    Robert A. Hirst

  2. Division of Anaesthesia, Critical Care and Pain Management, Leicester Royal Infirmar, University of Leicester, Lecester, UK

    Charlotte Harrison

  3. Department of Anesthesiology, University of Hirosaki School of Medicine, Hirosaki, Japan

    Kazuyoshi Hirota

  4. Department of Cardiovascular Sciences, Division of Anaesthesia, Critical Care, and Pain Management, Leicester Royal Infirmary, University of Leicester, Leicester, UK

    David G. Lambert

Authors
  1. Robert A. Hirst
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  2. Charlotte Harrison
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  3. Kazuyoshi Hirota
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  4. David G. Lambert
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Editor information

Editors and Affiliations

  1. Department of Cardiovascular Sciences, Division of Anaesthesia, Critical Care, and Pain Management, Leicester Royal Infirmary, Leicester, UK

    David G. Lambert

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© 2006 Humana Press Inc.

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Hirst, R.A., Harrison, C., Hirota, K., Lambert, D.G. (2006). Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2. In: Lambert, D.G. (eds) Calcium Signaling Protocols. Methods in Molecular Biology™, vol 312. Humana Press. https://doi.org/10.1385/1-59259-949-4:037

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  • DOI: https://doi.org/10.1385/1-59259-949-4:037

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-442-5

  • Online ISBN: 978-1-59259-949-3

  • eBook Packages: Springer Protocols

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