Abstract
Candida albicans is capable of forming biofilms on a variety of inert and biological surfaces. Cells in biofilms display phenotypic properties that are radically different from their free-floating planktonic counterparts, including their recalcitrance to antimicrobial agents. Consequently, Candida biofilm-associated infections are difficult to treat and to fully eradicate with standard antifungal therapy. Here, we describe a simple, fast, inexpensive and highly reproducible microtiter plate-based assay for the antifungal susceptibility testing of C. albicans biofilms. Because of its simplicity, compatibility with widely available 96-well microplate platform, high throughput, and automation potential, this assay represents an important tool towards the standardization of in vitro antifungal susceptibility testing of fungal biofilms.
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Ramage, G., López-Rib, J.L. (2005). Techniques for Antifungal Susceptibility Testing of Candida albicans Biofilms . In: Ernst, E.J., Rogers, P.D. (eds) Antifungal Agents. Methods in Molecular Medicine™, vol 118. Humana Press. https://doi.org/10.1385/1-59259-943-5:071
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DOI: https://doi.org/10.1385/1-59259-943-5:071
Publisher Name: Humana Press
Print ISBN: 978-1-58829-277-3
Online ISBN: 978-1-59259-943-1
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