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Natural Products and Antifungal Drug Discovery

  • Melissa R. Jacob
  • Larry A. Walker
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 118)

Abstract

The need for new antifungal agents continues, fueled by opportunistic infections in immunecompromised patients and by the development of resistance to existing agents. Natural products offer a virtually unlimited source of unique molecules and not only serve as a reservoir for new potential drugs and drug prototypes, but also for probes of fungal biology. In this chapter, whole-cell screening methods targeted for natural products are illustrated, including general microplate-based screening, bioautography, and mode of action studies, including the use of genetically altered fungal strains now available commercially.

Key Words

Antifungal susceptibility testing natural products whole-cell bioassay Candida albicans Cryptococcus neoformans Aspergillus Saccharomyces cerevisiae target-based screening bioautography tannins haploinsufficiency synergy 

Notes

Acknowledgments

The authors wish to thank Ameeta Agarwal, Xing-Cong Li, and Everett Jacob for their thorough review and suggestions. We also thank the Public Health Service, National Institute of Allergy and Infectious Diseases, grant number R01 AI27094 and the US Department of Agriculture Agricultural Research Service Specific Cooperative Agreement No. 58-6408-2-0009 for funding this work, and Dominique Sanglard (Institute of Microbiology, University Hospital, Lausanne, Switzerland) for supplying the S. cerevisiae strains used in Subheading 3.3.2.1.

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Melissa R. Jacob
    • 1
  • Larry A. Walker
    • 1
    • 2
  1. 1.National Center for Natural Products Research, Research Institute of Pharmaceutical SciencesUniversity of MississippiUniversity
  2. 2.Department of Pharmacology, School of PharmacyUniversity of MississppiUniversity

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