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Methods for Measuring TGF-β Using Antibodies, Cells, and Mice

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Fibrosis Research

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 117))

Abstract

The transforming growth factor (TGF)-βs are essential in pre- and postnatal development, differentiation and morphogenesis of higher organisms. Quantitation of the levels of TGF-β synthesis, secretion, and activation are crucial for grasping the mechanisms that control these events and ultimately control TGF-β action. Rather than presenting a single method, we describe several methods for measuring active TGF-β in different experimental situations. This is possible as a result of advances in transgenic mice technology that allow in vivo TGF-β measurements in addition to the more established in vitro approaches.

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Acknowledgments

This work was supported by grants from the National Institutes of Health.

Author information

Authors and Affiliations

  1. Department of Biochemistry and Cell Biology, SUNY at Stony Brook, Stony Brook, NY

    Vladimir Jurukovski

  2. Departments of Cell Biology and Medicine, New York University School of Medicine, New York, NY

    Branka Dabovic, Vesna Todorovic, Yan Chen & Daniel B. Rifkin

Authors
  1. Vladimir Jurukovski
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  2. Branka Dabovic
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  3. Vesna Todorovic
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  4. Yan Chen
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  5. Daniel B. Rifkin
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Editor information

Editors and Affiliations

  1. Feinberg School of Medicine, Northwestern University, Chicago, IL

    John Varga MD

  2. College of Physicians and Surgeons, Columbia University, New York, NY

    David A. Brenner MD

  3. University of Michigan Medical School, Ann Arbor, MI

    Sem H. Phan MD, PhD

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© 2005 Humana Press Inc.

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Jurukovski, V., Dabovic, B., Todorovic, V., Chen, Y., Rifkin, D.B. (2005). Methods for Measuring TGF-β Using Antibodies, Cells, and Mice. In: Varga, J., Brenner, D.A., Phan, S.H. (eds) Fibrosis Research. Methods in Molecular Medicine, vol 117. Humana Press. https://doi.org/10.1385/1-59259-940-0:161

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  • DOI: https://doi.org/10.1385/1-59259-940-0:161

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-479-1

  • Online ISBN: 978-1-59259-940-0

  • eBook Packages: Springer Protocols

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