Abstract
7q abnormalities are the most common cytogenetic or genetic aberrations found in splenic marginal zone lymphoma. The molecular methods whereby these regions of genetic loss can be characterized are discussed in this chapter. Emphasis is given to careful experimental design, for example sample purification and optimization, that is, ensuring that only target sequences are amplified. There also is discussion of result interpretation, pitfalls that may be encountered, and alternative visualization techniques that might be used.
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References
Jaffe, E. S., Harris, N. L., Stein, H., and Vardiman, J. W. (2001)Pathology and genetics of tumors of haematopoietic and lymphoid tissues, in WHO Classification of Tumors. WHO, Geneva.
Chacon, J. L., Mollegjo, M., Munox, E., Algara, P., Mateo, M., Lopez, L., et al. (2002) Splenic marginal zone lymphoma: clinical characteristics and prognostic factors in a series of 60 patients. Blood 100, 1648.
Algara, P., Mateo, M. S., Sanchez-Beato, M., Mollejo, M., Navas, I. C., Romero, L., et al. (2002) Analysis of the IgVH somatic mutations in splenic marginal zone lymphoma defines a group of unmutated cases with frequent 7q deletion and adverse clinical course. Blood 99, 1299.
Corcoran, M. M., Mould, S. J., Orchard, J. A., Ibbotson, R. E., Chapman, R. M., Boright, A. P., et al. (1999) Dysregulation of cyclin dependent kinase 6 expression in splenic marginal zone lymphoma through chromosome 7q translocations. Oncogene 18, 6271–6277.
Mateo, M., Mollejo, M., Villuendas, R., Algara, P., Sanchez-Beato, M., Martinez P., et al. (1999) 7q31–32 allelic loss is a frequent finding in splenic marginal zone lymphoma. Am. J. Pathol. 154, 1583–1589.
Jones M. H., Nakamura Y. (1992) Detection of loss of heterozygosity at the human TP53 locus using a dinucleotide repeat polymorphism. Genes Chromosomes Cancer 5, 89–90.
Tripputi P., Cassani B., Alfano R., Graziani D., Cigognini D., Doi P., et al. (1993) Detection of human chromosomes in somatic cell hybrids by PCR analysis. Cytogenet Cell Genet. 62, 1–4.
Corcoran M. M., Rasool O., Liu Y., Iyengar A., Grander D., Ibbotson R. E., et al. (1998) Detailed molecular delineation of 13q14.3 loss in B-cell chronic lymphocytic leukaemia. Blood 91, 1382–1390.
Pabst T., Schwaller J., Tobler A., Fey M. F. (1996) Detection of microsatellite markers in leukaemia using DNA from archival bone marrow smears. Br. J. Haematol. 95, 135–137.
Acknowledgments
The Bournemouth Leukaemia Fund and the Leukaemia Research Fund supported the work in the authors’ laboratory, and we thank Mary Tiller for her excellent technical assistance.
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© 2005 Humana Press Inc.
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Ibbotson, R.E., Parker, A.E., Oscier, D.G. (2005). Splenic Marginal Zone Lymphoma. In: Illidge, T., Johnson, P.W.M. (eds) Lymphoma. Methods in Molecular Medicine™, vol 115. Humana Press. https://doi.org/10.1385/1-59259-936-2:241
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DOI: https://doi.org/10.1385/1-59259-936-2:241
Publisher Name: Humana Press
Print ISBN: 978-1-58829-159-2
Online ISBN: 978-1-59259-936-3
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