Abstract
Serological analysis of recombinant complementary deoxyribonucleic acid (cDNA) expression libraries (SEREX) is a powerful approach to identify immunogenic cancer-associated proteins using antibodies that are naturally present in the serum of cancer patients. This technique involves the screening of a relevant cDNA expression library with patient serum that has been cleaned to remove any antibodies that may recognize bacterial and/or viral proteins. Once antigens have been identified and their reactivity has been confirmed with a second round of screening, the gene encoding the protein can be sequenced and identified. Antigens can then be screened with a panel of allogenic sera from other patients and control individuals. This identifies disease-specific antigens, which may be useful diagnostic markers or, alternatively, targets for immunotherapy. This chapter describes the SEREX methodology in full.
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Liggins, A.P., Guinn, B.A., Banham, A.H. (2005). Identification of Lymphoma-Associated Antigens Using SEREX. In: Illidge, T., Johnson, P.W.M. (eds) Lymphoma. Methods in Molecular Medicine™, vol 115. Humana Press. https://doi.org/10.1385/1-59259-936-2:109
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DOI: https://doi.org/10.1385/1-59259-936-2:109
Publisher Name: Humana Press
Print ISBN: 978-1-58829-159-2
Online ISBN: 978-1-59259-936-3
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