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Lymphoma pp 15–63Cite as

Molecular Diagnosis of Lymphoma

Outcome Prediction by Gene Expression Profiling in Diffuse Large B-Cell Lymphoma

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Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 115))

Abstract

This chapter describes the illness diffuse large B-cell lymphoma (DLBCL) and why research has and continues to focus on creating accurate predictors of response to treatment to allow individual risk assessment for a patient and individualization of treatment choice to maximize the chances of cure. Microarray technology has the promise to bring these objectives within reach. The first papers attempting to identify molecular signatures of response and outcome using microarray technology were generated using DLBCL samples and are described. The different types of microarray platform and data analysis tools are reviewed followed by a detailed step-by-step guide to data generation using the Affymetrix chip system from RNA extraction to laser scanning of the hybridized and stained chips.

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Author information

Authors and Affiliations

  1. St. Barthlomew’s Hospital, London, UK

    Kim Last, Silvana Debarnardi & T. Andrew Lister

Authors
  1. Kim Last
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  2. Silvana Debarnardi
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  3. T. Andrew Lister
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Editor information

Editors and Affiliations

  1. CRUK, Paterson Institute for Cancer Research, University of Manchester, Manchester, UK

    Tim Illidge

  2. Cancer Sciences Division, Southampton General Hospital, Southampton, UK

    Peter W. M. Johnson

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© 2005 Humana Press Inc.

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Last, K., Debarnardi, S., Lister, T.A. (2005). Molecular Diagnosis of Lymphoma. In: Illidge, T., Johnson, P.W.M. (eds) Lymphoma. Methods in Molecular Medicine™, vol 115. Humana Press. https://doi.org/10.1385/1-59259-936-2:015

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  • DOI: https://doi.org/10.1385/1-59259-936-2:015

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-159-2

  • Online ISBN: 978-1-59259-936-3

  • eBook Packages: Springer Protocols

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