Lymphoma pp 241-250 | Cite as

Splenic Marginal Zone Lymphoma

7q Abnormalities
  • Rachel E. Ibbotson
  • Anton E. Parker
  • David G. Oscier
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 115)


7q abnormalities are the most common cytogenetic or genetic aberrations found in splenic marginal zone lymphoma. The molecular methods whereby these regions of genetic loss can be characterized are discussed in this chapter. Emphasis is given to careful experimental design, for example sample purification and optimization, that is, ensuring that only target sequences are amplified. There also is discussion of result interpretation, pitfalls that may be encountered, and alternative visualization techniques that might be used.

Key Words

Splenic marginal zone lymphoma (SMZL) splenic lymphoma with villous lymphocytes (SLVL) 7q microsatellites loss of heterozygosity (LOH) 



The Bournemouth Leukaemia Fund and the Leukaemia Research Fund supported the work in the authors’ laboratory, and we thank Mary Tiller for her excellent technical assistance.


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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Rachel E. Ibbotson
    • 1
  • Anton E. Parker
    • 1
  • David G. Oscier
    • 2
  1. 1.Molecular Biology DepartmentRoyal Bournemouth HospitalBournemouthUK
  2. 2.Haematology DepartmentRoyal Bournemouth HospitalBournemouthUK

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