Abstract
Small interfering RNAs (siRNAs) mediate RNA interference (RNAi), providing a powerful new tool for gene silencing in a sequence-specific manner. RNAi is a useful tool for studying gene function and regulation, but it also has potential for therapeutic applications. In many cases, a long-term effect of RNAi is required, such as in human immunodeficiency virus (HIV)-1-infected individuals. Lentiviral vectors are able to transduce nondividing cells, with sustained long-term expression of genes contained in the vector backbone.
Keywords
- Enhanced Green Fluorescent Protein
- Transduction Efficiency
- Transfer Vector
- Vesicular Stomatitis Virus Glycoprotein
- shRNA Expression Cassette
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Acknowledgments
We thank Dr. Jiing-Kaun Yee for providing pHIV-7-GFP and the packaging plasmids. This work was supported by NIH grants AI29329 and AI42552 and HL074704 to J.J.R.
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© 2005 Humana Press Inc.
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Li, M., Rossi, J.J. (2005). Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic Cells. In: Carmichael, G.G. (eds) RNA Silencing. Methods in Molecular Biology™, vol 309. Humana Press. https://doi.org/10.1385/1-59259-935-4:261
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DOI: https://doi.org/10.1385/1-59259-935-4:261
Publisher Name: Humana Press
Print ISBN: 978-1-58829-436-4
Online ISBN: 978-1-59259-935-6
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