Modification of Human U1 snRNA for Inhibition of Gene Expression at the Level of Pre-mRNA
U1 snRNA is a component of the U1 snRNP complex, which contains seven common snRNP proteins and three specific U1 snRNP proteins (1). It initiates spliceosome association with pre-mRNA by defining the 3′ boundary of exons (2). As the splicing reaction proceeds, U1 snRNP and the other spliceosome components are sequentially released from the transcript (3).
KeywordsVortex Chloroform Electrophoresis CaCl2 Glutamine
This work was supported by a grant from PHS, NIH, R01-AR30426, and from the Osteogenesis Imperfecta Foundation.
- 14.Murphy, J. T., Skuzeski, J. T., Lund, E., Steinberg, T. H., Burgess, R. R., and Dahlberg, J. E. (1987) Functional elements of the human U1 RNA promoter. Identification of five separate regions required for efficient transcription and template competition. J. Biol. Chem. 262, 1795–1803.PubMedGoogle Scholar