RNA Silencing pp 321-331 | Cite as

Modification of Human U1 snRNA for Inhibition of Gene Expression at the Level of Pre-mRNA

  • Peng Liu
  • Mary Louise Stover
  • Alexander Lichtler
  • David W. Rowe
Part of the Methods in Molecular Biology™ book series (MIMB, volume 309)


U1 snRNA is a component of the U1 snRNP complex, which contains seven common snRNP proteins and three specific U1 snRNP proteins (1). It initiates spliceosome association with pre-mRNA by defining the 3′ boundary of exons (2). As the splicing reaction proceeds, U1 snRNP and the other spliceosome components are sequentially released from the transcript (3).


Mutagenic Primer PstI Site Terminal Exon Lauroyl Sarcosinate Bacterial Alkaline Phosphatase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by a grant from PHS, NIH, R01-AR30426, and from the Osteogenesis Imperfecta Foundation.


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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Peng Liu
    • 1
  • Mary Louise Stover
    • 1
  • Alexander Lichtler
    • 1
  • David W. Rowe
    • 1
  1. 1.Department of Genetics and Developmental BiologyUniversity of Connecticut Health CenterFarmington

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