An Optical Immunosensor for Pesticide Determination in Natural Waters

  • Sara Rodríguez-Mozaz
  • Maria J. López de Alda
  • Damia Barceló
Protocol
Part of the Methods in Biotechnology book series (MIBT, volume 19)

Abstract

The river analyzer (RIANA) is an optical immunosensor able to determine different types of organic pollutants in water samples. Interaction between fluorescently labeled antibody and analyte takes place on a solid-liquid interface and is detected by measuring the fluorescence. The RIANA integrates the optical part with a fluidics part in a compact unit, which is coupled to an autosampler, allowing the performance of automatic measurements within 15 min.

In this chapter, the use protocol for the sensor is described, and guidelines about experiment performance are provided. Up to three different analytes (in this case, the pesticides atrazine and isoproturon and the natural estrogen estrone) can be detected simultaneously. This multianalyte configuration allows reduction in time of analysis and in volume of sample and other reagents required. Different aspects, such as assay performance, cross-reactivity, matrix effects, or validation, are also considered.

Key Words

Environmental monitoring estrogens immunosensor multianalyte pesticides water 

Notes

Acknowledgments

This work has been supported by the Commission of the European Communities, AWACSS (EVK1-CT-2000-00045) and Ministerio de Ciencia y Tecnología (project PPQ 2000-3006-CE). Maria José López de Alda acknowledges a Ramon y Cajal contrac from the Spanish Ministry of Science and Technology.

References

  1. 1.
    Parellada, J., Narvaez, A., Lopez, M. A., et al. (1998) Amperometric immunosensors and enzyme electrodes for environmental applications. Anal. Chim. Acta 362, 47–57.CrossRefGoogle Scholar
  2. 2.
    Mallat, E., Barzen, C., Abuknesha, R., Gauglitz, G., and Barcelo, D. (2001) Fast determination of paraquat residues in water by an optical immunosensor and validation using capillary electrophoresis-ultraviolet detection. Anal. Chim. Acta 427, 165–171.CrossRefGoogle Scholar
  3. 3.
    Mallat, E., Barzen, C., Abuknesha, R., Gauglitz, G., and Barcelo, D. (2001) Part per trillion level determination of isoproturon in certified and estuarine water samples with a direct optical immunosensor. Anal. Chim. Acta 426, 209–216.CrossRefGoogle Scholar
  4. 4.
    Mallat, E., Barzen, C., Klotz, A., Brecht, A., Gauglitz, G., and Barcelo, D. (1999) River analyzer for chlorotriazines with a direct optical immunosensor. Environ. Sci. Technol. 33, 965–971.CrossRefGoogle Scholar
  5. 5.
    Klotz, A., Brecht, A., Barzen, C., et al. (1998) Immunofluorescence sensor for water analysis. Sens. Actuators B 51, 181–187.CrossRefGoogle Scholar
  6. 6.
    Barzen, C., Brecht, A., and Gauglitz, G. (2002) Optical multiple-analyte immunosensor for water pollution control. Biosens. Bioelectron. 17, 289–295.CrossRefPubMedGoogle Scholar
  7. 7.
    Coille, I., Reder, S., Bucher, S., and Gauglitz, G. (2002) Comparison of two fluorescence immunoassay methods for the detection of endocrine disrupting chemicals in water. Biomol. Eng. 18, 273–280.CrossRefPubMedGoogle Scholar
  8. 8.
    Kroger, S., Piletsky, S., and Turner, A. P. F. (2002) Biosensors for marine pollution research, monitoring and control. Mar. Pollut. Bull. 45, 24–34.CrossRefPubMedGoogle Scholar
  9. 9.
    Vo-Dinh, T., Fetzer, J., and Campiglia, A. D. (1998) Monitoring and characterization of polyaromatic compounds in the environment. Talanta 47, 943–969.CrossRefPubMedGoogle Scholar
  10. 10.
    Hennion, M.-C. and Barcelo, D. (1998) Strengths and limitations of immunoassays for effective and efficient use for pesticide analysis in water samples: a review. Anal. Chim. Actta 362, 3–34.CrossRefGoogle Scholar
  11. 11.
    Marco, M.-P. and Barcelo, D. (1996) Environmental applications of analytical biosensors. Meas. Sci. Technol. 7, 1547–1562.CrossRefGoogle Scholar
  12. 12.
    Rodriguez-Mozaz, S., Reder, S., Lopez de Alda, M., Gauglitz, G., and Barceló, D. (2004) Simultaneous determination of estrone, isoproturon and atrazine in natural waters by an optical immunosensor river analyser (RIANA). Biosens. Bioelectron. 19, 633–640.CrossRefPubMedGoogle Scholar
  13. 13.
    Reder, S., Dieterle, F., Jansen, H., Alcock, S., and Gauglitz, G. (2003) Multi-analyte assay for triazines using cross-reactive antibodies and neural networks. Biosens. Bioelectron. 19, 447–455.CrossRefPubMedGoogle Scholar

Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Sara Rodríguez-Mozaz
    • 1
  • Maria J. López de Alda
    • 2
  • Damia Barceló
    • 2
  1. 1.Department of Analytical Chemistry, Faculty of SciencesUniversity of CórdobaCórdobaSpain
  2. 2.Department of Environmental ChemistryIIQAB-CSICBarcelonaSpain

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