An Optical Immunosensor for Pesticide Determination in Natural Waters
Part of the
Methods in Biotechnology
book series (MIBT, volume 19)
The river analyzer (RIANA) is an optical immunosensor able to determine different types of organic pollutants in water samples. Interaction between fluorescently labeled antibody and analyte takes place on a solid-liquid interface and is detected by measuring the fluorescence. The RIANA integrates the optical part with a fluidics part in a compact unit, which is coupled to an autosampler, allowing the performance of automatic measurements within 15 min.
In this chapter, the use protocol for the sensor is described, and guidelines about experiment performance are provided. Up to three different analytes (in this case, the pesticides atrazine and isoproturon and the natural estrogen estrone) can be detected simultaneously. This multianalyte configuration allows reduction in time of analysis and in volume of sample and other reagents required. Different aspects, such as assay performance, cross-reactivity, matrix effects, or validation, are also considered.
Key WordsEnvironmental monitoring estrogens immunosensor multianalyte pesticides water
This work has been supported by the Commission of the European Communities, AWACSS (EVK1-CT-2000-00045) and Ministerio de Ciencia y Tecnología (project PPQ 2000-3006-CE). Maria José López de Alda acknowledges a Ramon y Cajal contrac from the Spanish Ministry of Science and Technology.
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