Abstract
This chapter describes methods for the production and use of antibody microarrays. The experimental methods are divided into three sections. The first gives information relating to the preparation and handling of antibodies and the production of microarrays. The second relates to the preparation of the samples, from either blood, tissue, or cell lysates. The third section describes methods for using the samples on antibody microarrays. Two related detection methods are described in which the proteins of a sample to be analyzed are labeled with a tag to allow detection after capture on antibody microarrays.
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Acknowledgments
This work was funded through the Van Andel Research Institute, the Early Detection Research Network (U01 CA84986), and the Michigan Economic Development Corporation.
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© 2005 Humana Press Inc.
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Haab, B.B. (2005). Multiplexed Protein Analysis Using Antibody Microarrays and Label-Based Detection. In: Joos, T.O., Fortina, P. (eds) Microarrays in Clinical Diagnostics. Methods in Molecular Medicine™, vol 114. Humana Press. https://doi.org/10.1385/1-59259-923-0:183
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DOI: https://doi.org/10.1385/1-59259-923-0:183
Publisher Name: Humana Press
Print ISBN: 978-1-58829-394-7
Online ISBN: 978-1-59259-923-3
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