Abstract
Virus removal filters (nanofilters) are specifically designed to remove viruses and other biomolecules through a size-exclusion mechanism. Nanofilters can be broadly classified as either direct (or normal) flow filtration (DFF) or tangential flow filtration (TFF). In the TFF mode, the material to be filtered is flowed across the membrane in a direction roughly parallel to the filtration matrix. This operational mode is analogous to that used with conventional ultrafiltration/diafiltration membranes. Product passage is facilitated by restricting the flow of the retentate relative to the feed, thereby applying backpressure to the system, which results in product migration through the membrane. In the DFF mode, the product is driven through the filter perpendicular to the filtration matrix. The main advantage to the TFF format is that a constant sweeping current (across the membrane surface) minimizes the opportunity for the membrane to be clogged by impurities in the product being nanofiltered. Nanofilter operation in the DFF mode offers several advantages over TFF, including flow rates that are generally higher, easier integration into a production environment, a simpler unit operation, and lower capital expenditure.
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Korneyeva, M., Rosenthal, S. (2005). Virus Removal by Nanofiltration. In: Smales, C.M., James, D.C. (eds) Therapeutic Proteins. Methods in Molecular Biology™, vol 308. Humana Press. https://doi.org/10.1385/1-59259-922-2:221
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DOI: https://doi.org/10.1385/1-59259-922-2:221
Publisher Name: Humana Press
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