Expression of Human Papillomavirus Type 16 L1 in Baculovirus Expression Systems

Abstract

With the great achievements of recombinant DNA technology, a variety of expression systems, including yeast, baculovirus, adenovirus, and attenuated Salmonella expression systems, have been used for the overexpression of recombinant proteins. Recombinant baculovirus-insect cell systems have become widely used because: (1) baculoviruses have a restricted host range, which is limited to specific invertebrate species. It is much safer to work with these viruses than most mammalian viruses. (2) Baculovirus has a large genome that accommodates large exogenous genes. Recombinant baculovirus can still propagate in insect cell lines or larvae from different insect species. (3) Recombinant proteins expressed by baculovirus are processed, modified, and targeted to their appropriate cellular locations, where they are functionally similar to their authentic counterparts.