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Pharmaceutical Proteins From Methylotrophic Yeasts

  • Eric C. de Bruin
  • Erwin H. Duitman
  • Arjo L. de Boer
  • Marten Veenhuis
  • Ineke G. A. Bos
  • C. Erik Hack
Part of the Methods in Molecular Biology™ book series (MIMB, volume 308)

Abstract

Because of their favorable properties, methylotrophic yeasts have become increasingly important as cell factories for the production of biomaterials, therapeutic proteins, and vaccines. As a eukaryote, yeast can perform most of the posttranslational modifications that are required to ensure the functionality and/or stability of recombinant human proteins, such as N- and O-linked glycosylation, phosphorylation, and formation of disulfide bonds. In contrast to other yeast systems, foreign genes can be expressed at high levels under control of strong inducible promoters derived from genes encoding proteins that are involved in methanol metabolism. Furthermore, heterologous proteins can be secreted at high levels into the culture medium, which, in combination with the fact that few endogenous proteins are secreted, significantly facilitates purification of the desired protein. Finally, as unicellular microorganisms, methylotrophic yeasts have major advantages in industrial fermentation.

Keywords

Heterologous Protein Heterologous Gene Methylotrophic Yeast AOX1 Gene Recombinant Human Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Eric C. de Bruin
    • 1
  • Erwin H. Duitman
    • 2
  • Arjo L. de Boer
    • 2
  • Marten Veenhuis
    • 2
  • Ineke G. A. Bos
    • 1
  • C. Erik Hack
    • 3
  1. 1.Department of ImmunopathologySanquin Research at CLBAmsterdamThe Netherlands
  2. 2.Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB)University of GroningenHarenThe Netherlands
  3. 3.Departments of Immunopathology and Clinical Chemistry, Sanquin Research at CLBVU Medical CentreAmsterdamThe Netherlands

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