Pharmaceutical Proteins From Methylotrophic Yeasts
Because of their favorable properties, methylotrophic yeasts have become increasingly important as cell factories for the production of biomaterials, therapeutic proteins, and vaccines. As a eukaryote, yeast can perform most of the posttranslational modifications that are required to ensure the functionality and/or stability of recombinant human proteins, such as N- and O-linked glycosylation, phosphorylation, and formation of disulfide bonds. In contrast to other yeast systems, foreign genes can be expressed at high levels under control of strong inducible promoters derived from genes encoding proteins that are involved in methanol metabolism. Furthermore, heterologous proteins can be secreted at high levels into the culture medium, which, in combination with the fact that few endogenous proteins are secreted, significantly facilitates purification of the desired protein. Finally, as unicellular microorganisms, methylotrophic yeasts have major advantages in industrial fermentation.
KeywordsGlycerol Agar Recombination Fluoride Proline
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