Abstract
Expanded T-cell clones in the peripheral blood of patients with multiple myeloma and smoldering myeloma are usually CD8 positive and persist over long periods, suggesting that they are the result of chronic antigenic stimulation. The presence of enlarged T-cell clones can be demonstrated as bands other than the germ-line bands on Southern blots probed for the T-cell receptor β gene (Vβ), or defined by anti-TCRVβ monoclonal antibody staining. However, the most sensitive way to demonstrate clonality within a population of T-cells is by analysis of the length of complementarity-determining region 3 of the rearranged TCR gene, followed by sequencing. Furthermore, my colleagues and I have previously shown that the CD57+ T-cells expressing the “expanded” TCRVβ are monoclonal or biclonal, whereas the CD57- cells are usually polyclonal.
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References
Raitakari M., Brown R. D., Sze D. M., et al. (2000) T-cell expansions in patients with multiple myeloma have a phenotype of cytotoxic T cells. Br. J. Haematol. 110, 203–209.
Sze D. M.-Y., Giesajtis G., Brown R. D., et al. (2001) Clonal cytotoxic T cells are expanded in myeloma and reside in the CD8+CD57+CD28-compartment. Blood 98, 2817–2827.
Brown R. D., Pope B., Murray A., et al. (2001) Dendritic cells from patients with myeloma renumerically normal but functionally defective as they fail to upregulate CD80 (B7-1) expression after huCD40LT stimulation due to inhibition by TGF-β1 and IL-10. Blood 98, 2992–2998.
Sze D. M.-Y., Brown R. D., Yuen E., et al. (2003) Clonal cytotoxic T cells in myeloma. Leuk. Lymphoma 44, 1667–1674.
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© 2005 Humana Press Inc.
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Sze, D.MY. (2005). Clonality Detection of Expanded T-Cell Populations in Patients With Multiple Myeloma. In: Brown, R.D., Ho, P.J. (eds) Multiple Myeloma. Methods in Molecular Medicine™, vol 113. Humana Press. https://doi.org/10.1385/1-59259-916-8:257
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DOI: https://doi.org/10.1385/1-59259-916-8:257
Publisher Name: Humana Press
Print ISBN: 978-1-58829-392-3
Online ISBN: 978-1-59259-916-5
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