Abstract
Patients with multiple myeloma have a clonal proliferation of malignant plasma cells, each with an identical rearrangement of immunogloblin heavy and light chain genes. When these unique sequences are determined, a valuable molecular tool is available that can been used to detect the presence of the malignant population. Previous methods have employed oligonucleotides derived from these sequences and allele-specific polymeraes chain reaction to detect clonality. The method described in this chapter uses mRNA in situ hybridization (ISH) to demonstrate the presence of individual malignant cells. Single-cell analysis using mRNA ISH provides opportunities that when combined with immunophenotyping offer a valuable new investigative tool.
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© 2005 Humana Press Inc.
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Brown, R.D., Ho, P.J. (2005). Identification of Malignant Plasma Cells by mRNA In Situ Hybridization. In: Brown, R.D., Ho, P.J. (eds) Multiple Myeloma. Methods in Molecular Medicine™, vol 113. Humana Press. https://doi.org/10.1385/1-59259-916-8:175
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DOI: https://doi.org/10.1385/1-59259-916-8:175
Publisher Name: Humana Press
Print ISBN: 978-1-58829-392-3
Online ISBN: 978-1-59259-916-5
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