Incomplete DJH Rearrangements

  • David González
  • Ramón Garcia-Sanz
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 113)

Abstract

Analysis of Ig genes in B-cell malignancies has become an essential method in molecular diagnosis, and polymerase chain reaction (PCR) amplification of Ig heavy chain gene (IgH) rearrangements is now widely used for detection of clonality and minimal residual disease (MRD). Although several different sensitive protocols are now available for PCR analysis of IgH genes, they are frequently hampered owing to the high rate of somatic hypermutation present in multiple myeloma (MM). We recently described a new approach using incomplete DJH rearrangements as an alternative target. About 60% of MM samples contain an incomplete DJH rearrangement, 90% of them lacking on somatic mutations. This approach allows resolution of problems derived from primer mismatches, making DJH rearrangement a reliable and sensitive target for detection of clonality and MRD investigation in MM.

Key Words

Multiple myeloma; polymerase chain reaction IGH rearrangements DJH rearrangements somatic hypermutation. 

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • David González
    • 1
  • Ramón Garcia-Sanz
    • 2
  1. 1.Department of Haemato-OncologyInstitute of Cancer ResearchSurreyUK
  2. 2.Haematology ServiceUniversity Hospital of SalamancaSalamancaSpain

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