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Protein-Ligand Interactions pp 385–413Cite as

Single-Molecule Study of Protein-Protein and Protein-DNA Interaction Dynamics

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 305))

Abstract

Protein-protein and protein-DNA interactions play critical roles in biological systems, and these interactions often involve complex mechanisms and inhomogeneous dynamics. Single-molecule spectroscopy is a powerful and complimentary approach to decipher such spatially and temporally inhomogeneous protein interaction systems, providing new information that are not obtainable from static structure analyses, thermodynamics characterization, and ensemble-averaged measurements. To illustrate the single-molecule spectroscopy and imaging technology and their applications on studying protein-ligand interactions, this chapter focuses on discussing two recent single-molecule spectroscopy studies on protein-protein interaction in cell signaling process and on protein-DNA interactions in DNA damage recognition process. Key Words: Single-molecule spectroscopy and imaging; single-molecule protein conformational dynamics; protein-protein interaction dynamics in cell signaling; protein-DNA recognition dynamics in DNA damage recognition and repair.

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Authors and Affiliations

  1. William R. Wiley Environmental Molecular Sciences Laboratory, Fundamental Science Division, Pacific Northwest National Laboratory, Richland, WA, USA

    H. Peter Lu

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  1. H. Peter Lu
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Editors and Affiliations

  1. Department of Biophysics, University of Ulm, Ulm, Germany

    G. Ulrich Nienhaus

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© 2005 Humana Press Inc., Totowa, NJ

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Lu, H.P. (2005). Single-Molecule Study of Protein-Protein and Protein-DNA Interaction Dynamics. In: Ulrich Nienhaus, G. (eds) Protein-Ligand Interactions. Methods in Molecular Biology, vol 305. Humana, Totowa, NJ. https://doi.org/10.1385/1-59259-912-5:385

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  • DOI: https://doi.org/10.1385/1-59259-912-5:385

  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-1-58829-372-5

  • Online ISBN: 978-1-59259-912-7

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