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Phenotypic Characterization of Blood Monocytes From HIV-Infected Individuals

  • Protocol
Human Retrovirus Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 304))

Summary

Monocytes play an important, yet only partly understood, role in HIV-1 pathogenesis. Two main subsets of peripheral blood monocytes have been described; the major subset of monocytes are phenotypically characterized as being CD14hi/CD16−, and a minor subset (5–15% of total monocytes in healthy individuals), which are CD14lo/CD16hi, have been reported to be expanded in HIV-infected individuals. These CD14lo/CD16hi monocytes differ from the majority of monocytes in a number of ways, including the molecules expressed on their surface and how they function. Here we describe a flow-cytometric assay to identify and compare the expression of a representative surface molecule (CCR5) on CD14hi/CD16− and CD14lo/CD16hi monocytes in small volumes of whole blood, and methods to isolate monocyte subsets by both fluorescence-activated cell sorting (FACS) and magnetic bead sorting.

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Author information

Authors and Affiliations

  1. Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia

    Philip J. Ellery & Suzanne M. Crowe

  2. Department of Medicine, Monash University, Clayton, Victoria, Australia

    Philip J. Ellery & Suzanne M. Crowe

Authors
  1. Philip J. Ellery
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  2. Suzanne M. Crowe
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Editor information

Editors and Affiliations

  1. Departments of Laboratory Medicine and Microbiology, University of Washington School of Medicine, Seattle, WA

    Tuofu Zhu MD

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© 2005 Humana Press Inc.

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Ellery, P.J., Crowe, S.M. (2005). Phenotypic Characterization of Blood Monocytes From HIV-Infected Individuals. In: Zhu, T. (eds) Human Retrovirus Protocols. Methods in Molecular Biology™, vol 304. Humana Press. https://doi.org/10.1385/1-59259-907-9:343

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  • DOI: https://doi.org/10.1385/1-59259-907-9:343

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-495-1

  • Online ISBN: 978-1-59259-907-3

  • eBook Packages: Springer Protocols

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