Abstract
Effective screening of new vaccines and immunotherapeutics requires assay methods that can provide quantitative measurement of cellular immune responses. The enzyme-linked immunospot (ELISPOT) is a sensitive technique for the detection of cytokine-producing cells at the single cell level. This assay is rapid and reproducible and permits the direct enumeration of low-frequency antigen-specific T-cells. This protocol describes in detail an interferon (IFN)-γ ELISPOT method for measuring antigen-specific murine CD8+ T-cells. Spleen cells from specific cytotoxic T-lymphocyte (CTL) peptide-primed mice are used source of CD8+T-cells to demonstrate the utility of this technique. The assay procedure is facilitated by the use of a ready-to-use IFN-γ ELISPOT assay kit and it can be adapted to other model systems in which CD8+ T-cell responses are monitored.
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Cole, G.A. (2005). Interferon-γ ELISPOT Assay for the Quantitative Measurement of Antigen-Specific Murine CD8+ T-Cells. In: Kalyuzhny, A.E. (eds) Handbook of ELISPOT. Methods in Molecular Biology™, vol 302. Humana Press. https://doi.org/10.1385/1-59259-903-6:191
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DOI: https://doi.org/10.1385/1-59259-903-6:191
Publisher Name: Humana Press
Print ISBN: 978-1-58829-469-2
Online ISBN: 978-1-59259-903-5
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