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Handbook of ELISPOT pp 3–14Cite as

ELISPOT Assay

A Personal Retrospective

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 302))

Abstract

In 1983, papers describing the enzyme-linked immunospot (ELISPOT) technique were published by two groups, the first description from a team in Perth, Western Australia, and the second, soon thereafter, from a group in Gothenburg, Sweden. Described here is my recollection of the background and circumstances that lead to the assay’s development within the Perth group. Included are the early studies in 1981 through early 1982 that were conducted to bring the assay to fruition—both setbacks and solutions, and finally some generally unknown but amusing insights into the naming of the ELISPOT assay by the Gothenburg group.

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Author information

Authors and Affiliations

  1. Stress and Immune Responses, Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN

    Jonathon D. Sedgwick

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  1. Jonathon D. Sedgwick
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Editor information

Editors and Affiliations

  1. R&D Systems Inc.,, Minneapolis, MN

    Alexander E. Kalyuzhny

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© 2005 Humana Press Inc., Totowa, NJ

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Sedgwick, J.D. (2005). ELISPOT Assay. In: Kalyuzhny, A.E. (eds) Handbook of ELISPOT. Methods in Molecular Biology™, vol 302. Humana Press. https://doi.org/10.1385/1-59259-903-6:003

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  • DOI: https://doi.org/10.1385/1-59259-903-6:003

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-469-2

  • Online ISBN: 978-1-59259-903-5

  • eBook Packages: Springer Protocols

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