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Synthetic Nanoscale Elements for Delivery of Materials Into Viable Cells

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NanoBiotechnology Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 303))

Abstract

Arrays of vertically aligned carbon nanofibers (VACNFs) provide structures that are well suited for the direct integration and manipulation of molecular-scale phenomena within intact, live cells. VACNFs are fabricated via a combination of microfabrication techniques and catalytic plasma-enhanced chemical vapor deposition. In this chapter, we discuss the synthesis of VACNFs and detail the methods for introducing these arrays into the intracellular domain of mammalian cells for the purpose of delivering large macromolecules, specifically plasmid DNA, on a massively parallel basis.

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Author information

Authors and Affiliations

  1. Molecular-Scale Engineering and Nanoscale Technologies Research Group, Oak Ridge National Laboratory, Oak Ridge, TN

    Timothy E. McKnight, Anatoli V. Melechko, Michael A. Guillorn, Vladimir I. Merkulov & Michael L. Simpson

  2. Electrical and Computer Engineering Department, University of Tennessee, Knoxville, TN

    Anatoli V. Melechko

  3. Thin Film and Nanostructured Materials Physics Group, Oak Ridge National Laboratory, Oak Ridge, TN

    Douglas H. Lowndes

  4. Materials Science and Engineering Department and Center for Environmental Biotechnology University of Tennessee, Knoxville, TN

    Michael L. Simpson

Authors
  1. Timothy E. McKnight
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  2. Anatoli V. Melechko
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  3. Michael A. Guillorn
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  4. Vladimir I. Merkulov
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  5. Douglas H. Lowndes
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  6. Michael L. Simpson
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Editor information

Editors and Affiliations

  1. The Department of Chemistry, Vanderbilt University, Nashville, TN

    Sandra J. Rosenthal  & David W. Wright  & 

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© 2005 Humana Press Inc.

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McKnight, T.E., Melechko, A.V., Guillorn, M.A., Merkulov, V.I., Lowndes, D.H., Simpson, M.L. (2005). Synthetic Nanoscale Elements for Delivery of Materials Into Viable Cells. In: Rosenthal, S.J., Wright, D.W. (eds) NanoBiotechnology Protocols. Methods in Molecular Biology™, vol 303. Humana Press. https://doi.org/10.1385/1-59259-901-X:191

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  • DOI: https://doi.org/10.1385/1-59259-901-X:191

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-276-6

  • Online ISBN: 978-1-59259-901-1

  • eBook Packages: Springer Protocols

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