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Recognition and Processing of Misfolded Proteins by PA700, the 19S Regulatory Complex of the 26S Proteasome

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Ubiquitin-Proteasome Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 301))

Summary

The 26S proteasome is composed of the core 20S proteasome in association with the 19S regulatory complex, or PA700. PA700 has multiple activities, including ATPase activity, polyubiquitin-chain binding activity, deubiquitination activity, chaperone-like activity, and substrate remodeling activity. The concerted action of these activities leads to efficient degradation of protein substrates by the 26S proteasome. In this chapter we describe protocols for purifying PA700 and the 20S complexes from bovine red cells and present methods to assay the chaperone-like activity and the substrate remodeling activity of PA700.

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Acknowledgments

This work was supported by grants from the Welch Foundation (to P. J. T.), MDA (to G. N. D.), NIH-DK46818 (to G. N. D.), and NIH-DK49835 (to P. J. T.).

Author information

Authors and Affiliations

  1. Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX

    Chang-Wei Liu, George N. DeMartino & Philip J. Thomas

  2. Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT

    Elizabeth Strickland

Authors
  1. Chang-Wei Liu
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  2. Elizabeth Strickland
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  3. George N. DeMartino
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  4. Philip J. Thomas
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Editor information

Editors and Affiliations

  1. Department od Medicine and the Carolina Cardiovascular Biology Center, University of North Carolina at Chapel Hill, Chapel Hill, NC

    Cam Patterson MD

  2. Department of Cell and Developmental Biology, University of North Carolona at Chapel Hill, Chapel Hill, NC

    Douglas M. Cyr PhD

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© 2005 Humana Press Inc.

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Liu, CW., Strickland, E., DeMartino, G.N., Thomas, P.J. (2005). Recognition and Processing of Misfolded Proteins by PA700, the 19S Regulatory Complex of the 26S Proteasome. In: Patterson, C., Cyr, D.M. (eds) Ubiquitin-Proteasome Protocols. Methods in Molecular Biology™, vol 301. Humana Press. https://doi.org/10.1385/1-59259-895-1:071

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  • DOI: https://doi.org/10.1385/1-59259-895-1:071

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-252-0

  • Online ISBN: 978-1-59259-895-3

  • eBook Packages: Springer Protocols

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