Abstract
Yeasts are the focus of much research, both in their role as pathogens and as biotechnically important organisms, and not the least in their role as model systems for eukaryotic cells. In particular, Saccharomyces cerevisiae has also been the object of several proteomics-related efforts. However, the preparation of protein extract from yeast is complicated by the presence of a cell wall of mainly chitin and glucans, which needs to be disrupted in the extraction process. Several methods presenting solutions to this problem, in connection with sample preparation for two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), have been described (1,2), in which the cell wall is broken either by vortexing in the presence of glass beads or by sonication.
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© 2005 Humana Press Inc., Totowa, NJ
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Norbeck, J. (2005). Preparation of Yeast Samples for 2-D PAGE. In: Walker, J.M. (eds) The Proteomics Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-890-0:027
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DOI: https://doi.org/10.1385/1-59259-890-0:027
Publisher Name: Humana Press
Print ISBN: 978-1-58829-343-5
Online ISBN: 978-1-59259-890-8
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