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Chemosensitivity: Volume II pp 197–231Cite as

Gene Expression Profiling to Characterize Anticancer Drug Sensitivity

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Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 111))

Abstract

This chapter presents a protocol for using cDNA microarrays to acquire gene expression pro files that characterize anticancer drug sensitivity. The protocol includes steps for drug exposure, RNA isolation, preparation of fluorescently labeled samples, microarray hybridization, data pro cessing, and data analysis. In addition to the detailed protocol, important experimental design issues are discussed, and some preliminary experiments are recommended.

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Author information

Authors and Affiliations

  1. Rebecca and John Moores UCSD Cancer Center, University of California-San Diego, La Jolla, CA, USA

    James K. Breaux & Gerrit Los

Authors
  1. James K. Breaux
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  2. Gerrit Los
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Editor information

Editors and Affiliations

  1. Garden State Cancer Center, Belleville, NJ, USA

    Rosalyn D. Blumenthal

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© 2005 Humana Press Inc.

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Breaux, J.K., Los, G. (2005). Gene Expression Profiling to Characterize Anticancer Drug Sensitivity. In: Blumenthal, R.D. (eds) Chemosensitivity: Volume II. Methods in Molecular Medicine™, vol 111. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-889-7:197

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  • DOI: https://doi.org/10.1385/1-59259-889-7:197

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-58829-586-6

  • Online ISBN: 978-1-59259-889-2

  • eBook Packages: Springer Protocols

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