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TAT-Mediated Protein Transduction

Delivering Biologically Active Proteins to the Heart

  • Protocol
Molecular Cardiology

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 112))

Abstract

TAT protein transduction is a novel method of delivering biologically active proteins into cells and tissues through the fusion of a protein transduction domain to the protein of interest. The present chapter outlines the methodology pertaining to the preparation of TAT-fusion proteins and how to efficiently transduce these proteins into cultured cells or isolated rat hearts perfused in Langendorff mode.

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Author information

Authors and Affiliations

  1. Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA

    Åsa B. Gustafsson & Roberta A. Gottlieb

  2. James Hogg iCAPTURE Centre for Cardiovascular and Pulmonary, Research St. Paul’s Hospital, Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada

    David J. Granville

Authors
  1. Åsa B. Gustafsson
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  2. Roberta A. Gottlieb
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  3. David J. Granville
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Editor information

Editors and Affiliations

  1. Departments of Medicine and Physiology and Functional Genomics, College of Medicine, University of Florida, Gainesville, FL

    Zhongjie Sun

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© 2005 Humana Press Inc.

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B. Gustafsson, Å., Gottlieb, R.A., Granville, D.J. (2005). TAT-Mediated Protein Transduction. In: Sun, Z. (eds) Molecular Cardiology. Methods in Molecular Medicine™, vol 112. Humana Press. https://doi.org/10.1385/1-59259-879-X:081

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  • DOI: https://doi.org/10.1385/1-59259-879-X:081

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-363-3

  • Online ISBN: 978-1-59259-879-3

  • eBook Packages: Springer Protocols

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