Abstract
Molecular cardiology is a new area of cardiovascular medicine that aims to apply molecular biological techniques for the mechanistic investigation, diag nosis, prevention, and treatment of cardiovascular disease. As an emerging discipline, it has changed our conceptual thinking of cardiovascular develop ment, disease etiology, and pathophysiology. Although molecular cardiology is still at a very early stage, it has opened a promising avenue for understanding and controlling cardiovascular disease. With the rapid development and appli cation of molecular biological techniques, scientists and clinicians are closer to curing heart diseases that were thought to be incurable 20 yr ago. There clearly is a need for a more thorough understanding of the molecular mecha nisms of cardiovascular diseases to promote the advancement of cell and gene therapy for heart diseases. This chapter briefly reviews state-of-the-art tech niques in the area of molecular cardiology.
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References
Schneider, M. D. and French, B. A. (1993) The advent of adenovirus: gene therapy for cardiovascular disease. Circulation 88, 1937ā1942.
Svensson, E. C., Marshall, D. J., Woodard, K., et al. (1999) Efficient and stable transduction of cardiomyocytes after intramyocardial injection or intracoronary per fusion with recombinant adeno-associated virus vectors. Circulation 99, 201ā20
Sakoda, T., Kasahara, N., Hamamori, Y., and Kedes, L. (1993) A high-titer lentiviral production system mediates efficient transduction of differentiated cells including beating cardiac myocytes. J. Mol. Cell. Cardiol. 31, 2037ā2047.
Stolberg, S. G. (1999) The biotech death of Jesse Gelsinger. New York Times, November 28.
Wang, W. and Sun, Z. (2004) eNOS gene delivery attenuates cold-induced hyper tension (CIH). FASEB J. 18, A1221, 823.4 (abstract).
Xia, H., Mao, Q., Paulson, H., and Davidson, B. L. (2002) siRNA-mediated gene silencing in vitro and in vivo. Nature Biotechnol. 20, 1006ā1010.
Huentelman, M. J., Teschemacher, A. G., Paton, J. F. R., Raizada, M. K., and Kasparov S. (2004) Evaluation of tetracycline response element (TRE)-based ge netic switches for control of gene expression in adeno-and lentiviral vectors. FASEB J. 18, A1221, 823.5 (abstract).
Pfeffer, J. M., Pfeffer, M. A., Fletcher, P. F., and Braunwarld, E. (1991) Progres sive ventricular remodeling in rat with myocardium infarction. Am. J. Physiol. 260, 1406ā1414.
Fukuda, K. (2003) Application of mesenchymal stem cells for the regeneration of cardiomyocyte and its use for cell transplantation therapy. Hum. Cell. 16, 83ā94.
Kand, H. J., Kim, H. S., Zhang, S. Y., et al. (2004) Effects of intracoronary infu sion of peripheral blood stem-cells mobilised with granulocyte-colony stimulat ing factor on left ventricular systolic function and restenosis after coronary stenting in myocardial infarction: the MAGIC cell randomised clinical trial. Lan cet 6, 751ā75
Ozbaran, M., Omay, S. B., Nalbantgil, S., et al. (2004) Autologous peripheral stem cell transplantation in patients with congestive heart failure due to ischemic heart disease. Eur. J. Cardiothorac. Surg. 25, 342ā350.
Ott, H. C., Bonaros, N., Marksteiner, R., et al. (2004) Combined transplantation of skeletal myoblasts and bone marrow stem cells for myocardial repair in rats. Eur. J. Cardiothorac. Surg. 25, 627ā634.
Menasche, P. (2004) Skeletal myoblast transplantation for cardiac repair. Expert. Rev. Cardiovasc. Ther. 2, 21ā28.
Murry, C. E., Soonpaa, M. H., Reinecke, H., et al. (2004) Haematopoietic stem cells do not transdifferentiate into cardiac myocytes in myocardial infarcts. Nature 428, 664ā668.
Basam, L. B., Wagers, A. J., Christensen, J. L., Kofidis, T., Weissman, I. L., and Robbins, R. C. (2004) Haematopoietic stem cells adopt mature haematopoietic fates in ischaemic myocardium. Nature 428, 668ā673.
Dowell, J. D., Rubart, M., Pasumarthi, K. B., Soonpaa, M. H., and Field, L. J. (2003) Myocyte and myogenic stem cell transplantation in the heart. Cardiovasc. Res. 58, 336ā350.
Mangi, A. A., Noiseux, N., Kong, D., et al. (2003) Mesenchymal stem cells modi fied with Akt prevent remodeling and restore performance of infracted hearts. Nature Med. 9, 1195ā1201.
Sun, Z., Cade, R., Fregly, M. J., and Rowland, N. E. (1997) Effect of chronic treatment with propranolol on the cardiovascular responses to chronic cold expo sure. Physiol. Behav. 62, 379ā384.
Sun, Z., Zhang, Z., and Cade, R. (2003) Angiotensinogen gege knockout delays and attenuates cold-induced hypertension. Hypertension 41, 322ā327.
Hamawaki, M., Coffman, T. M., Lashus, A., et al. (1998) Pressure-overload hy pertrophy is unabated in mice devoid of AT1A receptor. Am. J. Physiol. 274, H868āH873.
Sun, Z., Wang, X., and Cade, R. (2003) AT1A receptors are essential to the devel opment of cold-induced hypertension. Circulation 108, 421.
Green, D. L., Malhotra, A., and Scheuer, J. (1993) Angiotensin II increases car diac protein synthesis in adult rat heart. Am. J. Phsiol. 265, H238āH243.
Waeber, B. and Brunner, H. R. (1996) Cardiovascular hypertrophy: role of angio tensin II and bradykinin. J. Cardiovasc. Pharmacol. 27, S36āS40.
Ohtani, S., Fujiwara, H., Hasegawa, K., et al. (1997) Upregulated expression of angiotensin II type 1 receptor gene in human pathologic hearts. J. Card. Fail. 3, 303ā310.
Xiao, G., Mao S., Baumgarten, G., et al. (2001) Inducible activation of c-Myc in adult myocardium in vivo provokes cardiac myocyte hypertrophy and reactiva tion of DNA synthesis. Circ. Res. 89, 1122ā1129.
Kroumpouzou, E., Gomatos, I. P., Kataki, A., Karayannis, M., Dangas, G. D., and Toutouzas, P. (2003) Common pathways for primary hypertrophic and dilated cardiomyopathy. Hybrid. Hybridomics. 22, 41ā45.
Field, L. J. (1988) Atrial natriuretic factor-SV 40 Tantigen transgenes produce tumors and cardiac arrhythmias in mice. Science 239, 1029ā1033.
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Sun, Z. (2005). Introduction to Methods in Molecular Cardiology. In: Sun, Z. (eds) Molecular Cardiology. Methods in Molecular Medicineā¢, vol 112. Humana Press. https://doi.org/10.1385/1-59259-879-X:003
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DOI: https://doi.org/10.1385/1-59259-879-X:003
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