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Transgenic Mouse Models for Cardiac Dysfunction by a Specific Gene Manipulation

  • Gopal J. Babu
  • Muthu Periasamy
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 112)

Abstract

The sarcoplasmic reticulum Casn+2 ATPase (SERCA) plays a pivotal role in calcium cycling and the beat-to-beat function of the heart. Recent studies have shown that decreased expression and activity of SERCA are associated with end-stage heart failure in humans and in experimental animal models of heart failure. There has been considerable controversy over whether a decrease in SERCA level is a cause or effect of hypertrophy. To address directly whether alterations in SERCA levels modify calcium homeostasis and heart function, we have chosen to alter the SERCA protein expression genetically using transgenic and gene-targeted knockout mouse technology. This chapter describes the methodology for generation of mouse models that overexpress different SERCA isoforms and a SERCA2 knockout mouse model with de creased SERCA levels.

Key Words

SR Ca +2 ATPase αMHC promoter transgenic knockout mouse heart 

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Gopal J. Babu
    • 1
  • Muthu Periasamy
    • 1
  1. 1.Department of Physiology and Cell BiologyThe Ohio StateUniversityColumbusOH

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