Cardiac Gene Delivery Using DNA–Adenoviral Constructs

  • J. Mark Jones
  • Walter J. Koch
Protocol
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 112)

Abstract

In view of the limitations of current cardiac gene transfer techniques by direct myocardial injection, or via the coronary vasculature, we have been attempting to develop potentially clinically applicable methods. Selective catheterization of the coronary arteries has been performed, but the duration of exposure of the heart to the virus is limited. Present systems require high infusion pressure to inject the virus, which could result in endothelial or myocardial injury, and extracardiac transgene expression occurs.

Key Words

Gene transfer intracoronary adenoviral vector coronary catheterization cardiac surgery cardiopulmonary bypass cardiac-selective 

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • J. Mark Jones
    • 1
  • Walter J. Koch
    • 2
  1. 1.Department of Cardiac Surgery, Royal Victoria Hospital, Belfast, United Kingdomformerly Department of Surgery, Duke University Medical CenterDurhamNC
  2. 2.Center for Translational MedicineThomas Jefferson UniversityPhiladelphiaPA

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