Protein Identification by Mass Spectrometric Analyses of Peptides

  • Ashley Martin
Part of the Methods in Molecular Biology™ book series (MIMB, volume 298)


The focus of this chapter is the isolation and analysis of subsets of proteins or subproteomes. These methodologies can be applied to address fundamental questions in many areas of biological research. Protocols are provided for common techniques that include protein isolation from mammalian cells and the subsequent identification of peptides derived from endogenous proteins by the action of proteases.

Key Words

Mass spectrometry proteome SDS-PAGE trypsin digest cell lysis 


  1. 1.
    Patterson, S. D. and Aebersold, R. H. (2003) Proteomics: the first decade and beyond. Nat. Genet. (suppl.) 33, 311–323.PubMedCrossRefGoogle Scholar
  2. 2.
    Herbert, B. R., Harry, J. L., Packer, N. H., Gooley, A. A., Pedersen, S. K., and Williams, K. L. (2001) What place for polyacrylamide in proteomics? Trends Biotechnol. 19, S3–S9 Suppl. S.PubMedCrossRefGoogle Scholar
  3. 3.
    Harlow, E. and Lane, D. (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor Press, Cold Spring Harbor, NY.Google Scholar
  4. 5.
    Shevchenko, A., Wilm, M., Vorm, O., and Mann, M. (1996) Mass spectrometric sequencing of proteins from silver stained polyacrylamide gels. Analyt. Chem. 68, 850–858.CrossRefGoogle Scholar
  5. 6.
    Serru, V., Le Naour, F., Billard, M., et al. (1999) Selective tetraspan-integrin complexes (CD81/α4β1, CD151/α3β1, CD151/α6β1) under conditions disrupting tetraspan interactions. Biochem. J. 340, 103–111.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Ashley Martin
    • 1
  1. 1.Cancer Research UK Institute for Cancer StudiesUniversity of BirminghamBirmingham

Personalised recommendations