Abstract
The compelling need for early detection and chemical characterization of protein deposits in conformational disorders has always relied on immunohistochemical techniques performed on diagnostic biopsy specimens. Although the identity of the culprit can be assessed, the molecular nature of the defect still requires the analysis of the extracted material. The purpose of this chapter is to provide investigators with a method to extract and analyze—from minute residual diagnostic tissue biopsies—the deposited proteins. If done successfully, the technique will allow further investigation by molecular studies, while the patient is still being evaluated.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Gallo, G., Picken, M., Buxbaum, J., and Frangione, B. (1989) The spectrum of monoclonal immunoglobulin deposition disease associated with immunocytic dyscrasias. Seminars Hematol. 26, 234–245.
Kaplan, B., Vidal, R., Kumar, A., Ghiso, J., Frangione, B., and Gallo, G. (1997) Amino-terminal identity of co-existent amyloid and non-amyloid immunoglobulin κ?light chain deposits. A human disease to study alterations of protein conformation. Clin. Exp. Immunol. 110, 472–478.
Gallo, G., Goñi, F., Boctor, F., et al. (1996) Light chain cardiomyopathy. Structural analysis of the light chain tissue deposits. Am. J. Pathol. 148, 1397–1405.
Kaplan, B., Hrncic, R., Murphy, C., Gallo, G., Weiss, D., and Solomon, A. (1999) Microextraction and purification techniques applicable to chemical characterization of amyloid proteins in minute amounts of tissue. Methods Enzymol. 309, 67–81.
Vidal, R., Goñi, F., Stevens, F., et al. (1999) Somatic mutations of the L12a gene in V-κ1 light chain deposition disease. Potential effects on aberrant conformation and deposition. Am. J. Pathol. 155, 2009–2017.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Humana Press Inc.
About this protocol
Cite this protocol
Goñi, F., Gallo, G. (2005). Extraction and Chemical Characterization of Tissue-Deposited Proteins From Minute Diagnostic Biopsy Specimens. In: Sigurdsson, E.M. (eds) Amyloid Proteins. Methods in Molecular Biology™, vol 299. Humana Press. https://doi.org/10.1385/1-59259-874-9:261
Download citation
DOI: https://doi.org/10.1385/1-59259-874-9:261
Publisher Name: Humana Press
Print ISBN: 978-1-58829-337-4
Online ISBN: 978-1-59259-874-8
eBook Packages: Springer Protocols