Purification of Amyloid Protein AA Subspecies From Amyloid-Rich Human Tissues

  • Gunilla T. Westermark
  • Per Westermark
Part of the Methods in Molecular Biology™ book series (MIMB, volume 299)


Protein AA, the major amyloid fibril protein in reactive (secondary) systemic amyloidosis is derived from the acute phase reactant liver-produced apolipoprotein serum AA (SAA) by proteolytic cleavage, usually in the C-terminal half of the 104 amino acid residues long precursor. The cleavage points in SAA vary between patients and the deposited protein AA is often quite heterogeneous. In this chapter, we describe methods to extract amyloid fibrils and to purify protein AA by sequential gel filtration. Further purification of subspecies of protein AA is best achieved by the use of differences in charge and chromatofocusing is described as the method of choice. Analytic methods include sodium dodecylsulfate polyacrylamide gel electrophoresis and analytic isoelectric focusing.

Key Words

econdary amyloidosis apolipoprotein fibril gel filtration isoelectric point protein fragment isoelectric focusing chromatofocusing polybuffer AA-subtypes amyloid extraction 


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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Gunilla T. Westermark
    • 1
  • Per Westermark
    • 2
  1. 1.Department of Biomedicine and Surgery, Division of Cell BiologyLinköping UniversityLinköpingSweden
  2. 2.Department of Genetics and Pathology, Rudbeck LaboratoryUppsala UniversityUppsalaSweden

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