Abstract
Horseradish peroxidase is coupled to IgG antibody in a two-step procedure. In the first step monosaccharide residues in the enzyme are oxidized with periodate to produce aldehyde groups. Then, in the second step, the aldehyde groups are allowed to react with amino groups in the IgG antibody. The Schiff bases formed are reduced and the conjugate is purified by gel filtration.
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References
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© 2005 Humana Press Inc., Totowa, NJ
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Wisdom, G.B. (2005). Conjugation of Antibodies to Horseradish Peroxidase. In: Burns, R. (eds) Immunochemical Protocols. Methods In Molecular Biology™, vol 295. Humana Press. https://doi.org/10.1385/1-59259-873-0:127
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DOI: https://doi.org/10.1385/1-59259-873-0:127
Publisher Name: Humana Press
Print ISBN: 978-1-58829-274-2
Online ISBN: 978-1-59259-873-1
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