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Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments

  • Protocol
Immunochemical Protocols

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 295))

Abstract

A major source of human antibodies are phage display libraries, which are constructed from various genetic sources. Antibodies are expressed as scFV and Fab antibody fragments using various vector systems. This review offers a comprehensive overview of M13 phage display antibody vectors and discusses their applications.

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Author information

Authors and Affiliations

  1. Department of Biotechnology, Institute of Biochemistry and Biotechnology, Carolo-Wilhelmina Technical University, Braunschweig, Germany

    Michael Hust

  2. Professor of Biotechnology, Institute of Biochemistry and Biotechnology, Carolo-Wilhelmina Technical University, Braunschweig, Germany

    Stefan Dübel

Authors
  1. Michael Hust
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  2. Stefan Dübel
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Editors and Affiliations

  1. Antibody Unit, Scottish Agricultural Science Agency, Edinburgh, Scotland

    Robert Burns

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© 2005 Humana Press Inc., Totowa, NJ

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Hust, M., Dübel, S. (2005). Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments. In: Burns, R. (eds) Immunochemical Protocols. Methods In Molecular Biology™, vol 295. Humana Press. https://doi.org/10.1385/1-59259-873-0:071

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  • DOI: https://doi.org/10.1385/1-59259-873-0:071

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-274-2

  • Online ISBN: 978-1-59259-873-1

  • eBook Packages: Springer Protocols

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