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Conjugation of Antibodies to Biotin

  • James D. Hirsch
  • Rosaria P. Haugland
Part of the Methods In Molecular Biology™ book series (MIMB, volume 295)

Abstract

Antibodies can be conjugated to biotin by a number of chemical means. They can then be used in immunochemical procedures in conjunction with secondary reagents coupled to biotin-binding protein proteins such as avidin.

Key Words

Biotin Avidin Antibody Labeling Affinity Fab Fragments Fluorescence 

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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • James D. Hirsch
    • 1
  • Rosaria P. Haugland
    • 1
  1. 1.Molecular Probes IncEugeneOR

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