Production of Polyclonal Antibodies in Rabbits

  • David C. Hancock
  • Nicola J. OReilly
Part of the Methods In Molecular Biology™ book series (MIMB, volume 295)


The generation of polyclonal antibodies to an antigen of interest is an important technique applicable to many areas of biological research. In this chapter, we describe a basic immunization procedure designed to generate polyclonal antisera in rabbits and two methods that are commonly employed in the subsequent preliminary characterization of antipeptide antibodies raised in this way.

Key Words

Antibody antigen polyclonal immunogen immunization peptide ELISA 


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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • David C. Hancock
    • 1
  • Nicola J. OReilly
    • 2
  1. 1.Signal Transduction Laboratory, Cancer Research UKLondon Research Institute LaboratoriesUK
  2. 2.Cancer Research UKLondon Research Institute LaboratoriesUK

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