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Enzyme-Linked Immunosorbent Assay

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Book cover Medical Biomethods Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

The enzyme-linked immunosorbent assay (ELISA) is typically used to detect and quantify antigen within biological fluids. Among the ELISA’s attributes are a very high level of sensitivity and robustness as well as being an extremely cost-effective assay that can be performed with only basic laboratory equipment. Having been developed over 30 yr ago (1), the ELISA remains an assay of choice for many routine medical and veterinary diagnostic assays, as well as being a vital assay in basic science research.

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Author information

Authors and Affiliations

  1. Division of Oral Biology, University of Medicine and Dentistry of New Jersey, Newark, NJ

    William J. Jordan

Authors
  1. William J. Jordan
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Editor information

Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    John M. Walker  & Ralph Rapley  & 

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© 2005 Humana Press Inc., Totowa, NJ

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Jordan, W.J. (2005). Enzyme-Linked Immunosorbent Assay. In: Walker, J.M., Rapley, R. (eds) Medical Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-870-6:419

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  • DOI: https://doi.org/10.1385/1-59259-870-6:419

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-288-9

  • Online ISBN: 978-1-59259-870-0

  • eBook Packages: Springer Protocols

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