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Probe Design, Production, and Applications

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Medical Biomethods Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

A probe is a nucleic acid molecule (single-stranded DNA or RNA) with a strong affinity with a specific target (DNA or RNA sequence). Probe and target base sequences must be complementary to each other, but depending on conditions, they do not necessarily have to be exactly complementary. The hybrid (probe-target combination) can be revealed when appropriate labeling and detection systems are used. Gene probes are used in various blotting and in situ techniques for the detection of nucleic acid sequences. In medicine, they can help in the identification of microorganisms and the diagnosis of infectious, inherited, and other diseases.

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Author information

Authors and Affiliations

  1. CABI Bioscience UK Centre, Egham, Surrey, UK

    Marilena Aquino de Muro

Authors
  1. Marilena Aquino de Muro
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Editor information

Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    John M. Walker  & Ralph Rapley  & 

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© 2005 Humana Press Inc., Totowa, NJ

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de Muro, M.A. (2005). Probe Design, Production, and Applications. In: Walker, J.M., Rapley, R. (eds) Medical Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-870-6:013

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  • DOI: https://doi.org/10.1385/1-59259-870-6:013

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-288-9

  • Online ISBN: 978-1-59259-870-0

  • eBook Packages: Springer Protocols

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