Analysis of Chromosomal Translocations

  • Andreas Hochhaus

Abstract

Chromosomal translocations were the first target for the specific detection of residual tumor cells in bone marrow and peripheral blood. Some types of leukemia are regularly or generally associated with translocations. In chronic myelogenous leukemia (CML) and a proportion of patients with acute lymphoblastic leukemia (ALL), the t(9;22)(q34;q11) translocation leads to the fusion of the ABL gene with part of the BCR gene. Locating the primers such that the polymerase chain reaction (PCR) product spans the fusion point makes it possible to amplify sequences of the hybrid gene specifically (see Chapter 6). Because the breakpoints are better defined at the RNA level than at the DNA level, the primary target is the RNA, which is transcribed into cDNA by reverse transcription. (RT-PCR). In this way, a single leukemic cell can be detected from among 106 normal bone marrow or peripheral blood cells. Comparable approaches have been applied to other fusions such as the PML-RARα fusion gene in acute promyelocytic leukemia (APL). The presence of a few cells with phenotypic or genotypic features of leukemia cells after therapeutic regimens such as stem cell transplantation is know as “minimal residual disease” (MRD).

Keywords

Migration Tyrosine Leukemia Agarose Polyacrylamide 

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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Andreas Hochhaus
    • 1
  1. 1.Medizinische KlinikFakultät für Klinische Medizin Mannheim der Universität HeidelbergMannheimGermany

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