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Forensic DNA Typing Protocols pp 67–81Cite as

Y Chromosome STR Typing

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 297))

Abstract

Because of their unique transmission properties and male specificity, markers located on the nonrecombining region of the Y chromosome (NRY) have become an important tool in forensic investigation. In the past few years, more than 50 polymorphic Y chromosome-specific short tandem repeats (STRs) have been described and a set of 9 loci were selected, considered as the minimal haplotype included in the forensic databases, Y-STR Haplotype Reference Databases, the largest now available online (http:// www.yhrd.org). Here, we describe a multiplex amplification strategy developed for ABI platforms to amplify the nine loci included in the minimal haplotype (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393) as well as a second set of markers (DYS437, DYS438, DYS439, DYS460, DYS461, GATA A10, GATA C4, and GATA H4) used to improve the minimal haplotype diversity and, hence, the discrimination power. Together, these 17 Y-STRs can be used to define highly discriminative haplotypes, providing a powerful tool in male lineage identification.

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Author information

Authors and Affiliations

  1. Instituto de Patologia e Imunologia Molecular da Universidade do Porto, Porto, Portugal

    Leonor Gusmão & Cíntia Alves

Authors
  1. Leonor Gusmão
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  2. Cíntia Alves
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Editor information

Editors and Affiliations

  1. Institute of Legal Medicine, University of Santiago de Compostela, Galicia, Spain

    Angel Carracedo

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© 2005 Humana Press Inc., Totowa, NJ

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Gusmão, L., Alves, C. (2005). Y Chromosome STR Typing. In: Carracedo, A. (eds) Forensic DNA Typing Protocols. Methods in Molecular Biology, vol 297. Humana Press. https://doi.org/10.1385/1-59259-867-6:067

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  • DOI: https://doi.org/10.1385/1-59259-867-6:067

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-264-3

  • Online ISBN: 978-1-59259-867-0

  • eBook Packages: Springer Protocols

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