Abstract
Dendritic cells (DC) can either be generated from progenitors such as stem cells or CD 14+ monocytes, or isolated directly from the blood. Blood-derived DC are present as at least two distinct populations-myeloid and plasmacytoid DC. Here we describe methods for the clinical grade isolation of blood DC and DC precursors using the CliniMACS™. We describe the isolation of ultra-pure monocytes in order to generate large numbers of monocyte-derived DC, and also new methods for the direct isolation of blood DC. Isolation of blood DC in large numbers means that natural DC with different properties can be investigated for their clinical function for the first time.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Banchereau, J. (1997) Dendritic cells: therapeutic potentials. Transfus Sci. 18, 313–326.
Dzionek, A., Fuchs, A., Schmidt, P., et al. (2000) BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165,6037–6046.
Dzionek, A., Inagaki, Y., Okawa, K., et al. (2002) Plasmacytoid dendritic cells: from specific surface markers to specific cellular functions. Hum. Immunol. 63, 1133–1148.
Fay, J. (1998) Dendritic cells in the treatment of cancer. Baylor Univ. Med. Cent. Proc.. 11(4), 217–219.
Pickl, W. F., Majdic, O., Kohl, P., et al. (1996) Molecular and functional characteristics of dendritic cells generated from highly purified CD 14+peripheral blood monocytes. J.Immunol 157, 3850–3859.
Thurner, B., Röder, C., Dieckmann, D., et al. (1999) Generation of large numbers of fully mature and stable dendritic cells from leukapheresis products for clinical application. J.Immunol. Meth. 223, 1–15.
Cella, M., Facchetti, F., Lanzavecchia, A., and Colonna, M. (2000) Plasmacytoid dendritic cells activated by influenza virus and CD40L drive a potent TH1 polarization. Nat.Immunol 1,305–310.
Cella, M., Jarrossay, D., Facchetti, F., et al. (1999) Plasmacytoid monocytes migrate to inflamed lymph nodes and produce large amounts of type I interferon. Nat. Med. 5, 919–923.
Fong, L., Hou, Y., Rivas, A., et al. (2001) Altered peptide ligand vaccination with Flt3 ligand expanded dendritic cells for tumor immunotherapy. PNAS 98, 8809–8814.
Ito, T., Inaba, M., Inaba, K., et al. (1999) A CD1a+/CD11c+subset of human blood dendritic cells is a direct precursor of Langerhans cells. J. Immunol. 163, 1409–1419.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Humana Press Inc.
About this protocol
Cite this protocol
Campbell, J.D.M. et al. (2005). Isolation and Generation of Clinical-Grade Dendritic Cells Using the CliniMACS System. In: Ludewig, B., Hoffmann, M.W. (eds) Adoptive Immunotherapy: Methods and Protocols. Methods in Molecular Medicine™, vol 109. Humana Press. https://doi.org/10.1385/1-59259-862-5:055
Download citation
DOI: https://doi.org/10.1385/1-59259-862-5:055
Publisher Name: Humana Press
Print ISBN: 978-1-58829-406-7
Online ISBN: 978-1-59259-862-5
eBook Packages: Springer Protocols