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Isolation and Culture of Human Osteoblasts

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Human Cell Culture Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 107))

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Abstract

Bone is a complex tissue that contains at least four different cell types of the osteoblastic lineage. (1) Active osteoblast-a plump, polarized, cuboidal cell rich in organelles involved in the synthesis and secretion of matrix proteins. (2) Osteocyte-an osteoblast with low metabolic activity that has been engulfed in matrix during bone formation and entombed in lacunae. (3) Bone-lining cell-osteoblasts that have avoided entombment in lacunae and lose their prominent synthetic function; these cells cover most of the bone surfaces in mature bone. (4) Preosteoblast-a fibroblastic proliferative cell with osteogenic capacity. In addition, bone contains cells of a distinct lineage, the osteoclast (reviewed in Ch. 4).

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Author information

Authors and Affiliations

  1. Human Bone Cell Research Group, Department of Human Anatomy & Cell Biology, University of Liverpool, UK

    Alison Gartland, Katherine A. Buckley, Jane P. Dillon & James A. Gallagher

  2. UK Centre for Tissue Engineering, Department of Clinical Engineering, University of Liverpool, UK

    Judith M. Curran & John A. Hunt

Authors
  1. Alison Gartland
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  2. Katherine A. Buckley
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  3. Jane P. Dillon
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  4. Judith M. Curran
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  5. John A. Hunt
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  6. James A. Gallagher
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Editors and Affiliations

  1. Southampton, UK

    Joanna Picot

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© 2005 Humana Press Inc.

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Gartland, A., Buckley, K.A., Dillon, J.P., Curran, J.M., Hunt, J.A., Gallagher, J.A. (2005). Isolation and Culture of Human Osteoblasts. In: Picot, J. (eds) Human Cell Culture Protocols. Methods in Molecular Medicine™, vol 107. Humana Press. https://doi.org/10.1385/1-59259-861-7:029

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  • DOI: https://doi.org/10.1385/1-59259-861-7:029

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-222-3

  • Online ISBN: 978-1-59259-861-8

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