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Cell Migration pp 335-345 | Cite as

Use of Multiphoton Imaging for Studying Cell Migration in the Mouse

  • Andrea Flesken-Nikitin
  • Rebecca M. Williams
  • Warren R. Zipfel
  • Watt W. Webb
  • Alexander Yu. Nikitin
Part of the Methods in Molecular Biology™ book series (MIMB, volume 294)

Abstract

We describe a method for studying cell motility in the living mouse using multiphoton microscopy. The procedure consists of mouse anesthesia, labeling of target cells with enhanced green fluorescent protein by infection with recombinant adenovirus, implantation of beads carrying chemoattractant, preparation of the mouse for imaging, and imaging of individual cell motions via multiphoton microscopy. Two-photon fluorescence excitation of enhanced green fluorescent protein allows visualization of cells within the dermis, whereas second harmonic generation (a non-linear scattering process) allows a simultaneous detailed definition of the dermis structure.

Key Words

Cell motility live imaging multiphoton microscopy mouse second harmonic generation stromal cells intradermal tissues 

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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Andrea Flesken-Nikitin
    • 1
  • Rebecca M. Williams
    • 2
  • Warren R. Zipfel
    • 1
  • Watt W. Webb
    • 3
  • Alexander Yu. Nikitin
    • 1
  1. 1.Department of Biomedical SciencesCornell UniversityIthaca
  2. 2.Department of Applied and Engineering PhysicsCornell UniversityIthaca
  3. 3.Department of Cell BiologyUniversity of VirginiaCharlottesville

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