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Nanosystems for Biosensing

Multianalyte Immunoassay on a Protein Chip

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Protein Nanotechnology

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 300))

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Summary

This chapter describes the construction of addressable two-dimensional (2D) microarrays via the random fluidic self-assembly of metallic particles and the use of these arrays as platforms for constructing protein chips for bioassays. These arrays will be useful as platforms for constructing protein chips for bioassays in a broad range of applications. The basic units in the assembly are microfabricated particles, which carry a straightforward visible code, and the corresponding array template patterned on a glass substrate. On one face, the particles consist of a hydrophobic and magnetic Ni-polytetrafluoroethylene (Ni-PTFE) composite layer; the other face has a gold layer that was modified for biomolecular attachment. We use photoresist patterning to create an array template with spatially discrete microwells into which an Ni-PTFE hydrophobic composite layer and a hydrophobic photoadhesive coating are electrodeposited. After biomaterial attachment and binding processes in bulk, the particles are randomly self-assembled onto the lubricated bonding sites on the chip substrate. This self-assembly process is driven by a combination of magnetic, hydrophobic, and capillary interactions. The encoding symbol carried by each particle is used to identify the target attached to the particle surface. This model system demonstrates the utility of the protein chip array for conducting simultaneous multianalyte immunoassays of human immunoglobulins (IgA, IgG, and IgM).

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Acknowledgments

We thank Prof. R. B. DiGiovanni and J. A. Steeh for editing assistance. This work was partially funded by a grant-in-aid for Scientific Research Priority Area (A) of MEXT, Research for Future Program from the JSPS, and the 21st Century Center of Excellence Program on Technology Creation Based on Knowledge Science at JAIST.

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Authors and Affiliations

  1. School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa, Japan

    Eiichi Tamiya, Zheng-liang Zhi & Yasutaka Morita

  2. Center for Strategic Development of Science and Technology (the 21st Century Center of Excellence Program), Japan Advanced Institute of Science and Technology, Ishikawa, Japan

    Quamrul Hasan

Authors
  1. Eiichi Tamiya
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  2. Zheng-liang Zhi
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  3. Yasutaka Morita
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  4. Quamrul Hasan
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Editor information

Editors and Affiliations

  1. Center for Advanced Biomedical Photonics, Oak Ridge National Laboratory, Oak Ridge, TN

    Tuan Vo-Dinh (Director) (Director)

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© 2005 Humana Press Inc., Totowa, NJ

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Tamiya, E., Zhi, Zl., Morita, Y., Hasan, Q. (2005). Nanosystems for Biosensing. In: Vo-Dinh, T. (eds) Protein Nanotechnology. Methods in Molecular Biology™, vol 300. Humana Press. https://doi.org/10.1385/1-59259-858-7:369

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  • DOI: https://doi.org/10.1385/1-59259-858-7:369

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-310-7

  • Online ISBN: 978-1-59259-858-8

  • eBook Packages: Springer Protocols

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