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Construction of Ordered Protein Arrays

  • Protocol
Book cover Protein Nanotechnology

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 300))

Summary

Artificially ordered protein arrays provide a facile approach to a variety of problems in biology and nanoscience. Current demonstration systems use either nucleic acid tethers or methyltransferase fusions in order to target proteins or peptides of interest to nucleic acid scaffolds. These demonstrations point to the large number of useful devices and assemblies that can be envisioned using this approach, including smart biological probes and drug delivery systems. In principle, these systems are now capable of imitating the earliest forms of prebiotic organisms and can be expected to reach the complexity of a small virus in the near future. Third-generation methyltransferase inhibitors provide an example of a smart chemotherapeutics that can be constructed with this approach. We describe the use of mechanistic enzymology, computer-aided design, and microfluidic chip-based capillary electrophoresis in assessing the final assembly and testing of designs of this type.

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Author information

Authors and Affiliations

  1. Kaplan Clinical Research Laboratory, City of Hope National Medical Center, Duarte, CA

    Jarrod Clark, Taras Shevchuk, Piotr M. Swiderski, Rajesh Dabur, Laura E. Crocitto & Steven S. Smith

  2. Laboratory of Plant Biotechnology, Branch of Shemyakin and Ovchinnikor Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow, Russia

    Yaroslav I. Buryanov

Authors
  1. Jarrod Clark
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  2. Taras Shevchuk
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  3. Piotr M. Swiderski
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  4. Rajesh Dabur
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  5. Laura E. Crocitto
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  6. Yaroslav I. Buryanov
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  7. Steven S. Smith
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Editors and Affiliations

  1. Center for Advanced Biomedical Photonics, Oak Ridge National Laboratory, Oak Ridge, TN

    Tuan Vo-Dinh (Director) (Director)

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© 2005 Humana Press Inc., Totowa, NJ

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Clark, J. et al. (2005). Construction of Ordered Protein Arrays. In: Vo-Dinh, T. (eds) Protein Nanotechnology. Methods in Molecular Biology™, vol 300. Humana Press. https://doi.org/10.1385/1-59259-858-7:325

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  • DOI: https://doi.org/10.1385/1-59259-858-7:325

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-310-7

  • Online ISBN: 978-1-59259-858-8

  • eBook Packages: Springer Protocols

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