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Polo-Like Kinase-1

Activity Measurement and RNAi-Mediated Knockdown

  • Protocol
Cell Cycle Control

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 296))

Abstract

Polo-like kinase-1 (Plk-1) is an important cell cycle regulatory kinase that has been implicated in a multitude of cell cycle events. In this chapter we review those multiple functions of Plk-1 and describe the methods routinely used in our laboratory to purify Plk-1 from cellular lysates and measure Plk-1 kinase activity in vitro. In addition, we describe a method to analyze cell cycle progression after depletion of Plk-1 by RNA-interference in tissue culture cells.

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Authors
  1. Marcel A. T. M. van Vugt
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  2. René H. Medema
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Editor information

Editors and Affiliations

  1. MRC Radiation and Genome Stability Unit, Harwell, Didcot, UK

    Tim Humphrey

  2. School of Pharmacy, University of Reading Whiteknights, Reading, UK

    Gavin Brooks

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© 2005 Humana Press Inc.

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van Vugt, M.A.T.M., Medema, R.H. (2005). Polo-Like Kinase-1. In: Humphrey, T., Brooks, G. (eds) Cell Cycle Control. Methods in Molecular Biology™, vol 296. Humana Press. https://doi.org/10.1385/1-59259-857-9:355

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  • DOI: https://doi.org/10.1385/1-59259-857-9:355

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-144-8

  • Online ISBN: 978-1-59259-857-1

  • eBook Packages: Springer Protocols

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