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Forkhead (FOX) Transcription Factors and the Cell Cycle

Measurement of DNA Binding by FoxO and FoxM Transcription Factors

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Cell Cycle Control

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 296))

Abstract

Certain forkhead (FOX) transcription factors have been shown to play an intrinsic role in controlling cell cycle progression. In particular, the FoxO subclass has been shown to regulate cell cycle entry and exit, whereas the expression and activity of FoxM 1 is important for the correct coupling of DNA synthesis to mitosis. In this chapter, I describe a method for measuring FoxO and FoxM1 transcription factor DNA binding in nuclear extracts from mammalian cells.

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Authors
  1. Katrina A. Bicknell
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Editor information

Editors and Affiliations

  1. MRC Radiation and Genome Stability Unit, Harwell, Didcot, UK

    Tim Humphrey

  2. School of Pharmacy, University of Reading Whiteknights, Reading, UK

    Gavin Brooks

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© 2005 Humana Press Inc.

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Bicknell, K.A. (2005). Forkhead (FOX) Transcription Factors and the Cell Cycle. In: Humphrey, T., Brooks, G. (eds) Cell Cycle Control. Methods in Molecular Biology™, vol 296. Humana Press. https://doi.org/10.1385/1-59259-857-9:247

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  • DOI: https://doi.org/10.1385/1-59259-857-9:247

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-144-8

  • Online ISBN: 978-1-59259-857-1

  • eBook Packages: Springer Protocols

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